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探讨不同毒力结核分枝杆菌感染对小鼠肺泡巨噬细胞转铁蛋白受体(TfR)和铁蛋白(Fn)表达的影响及其时相性变化。利用制备的卡介苗(以下简称BCG)和结核分枝杆菌国际标准强毒株H37Rv株(以下简称H37Rv株)悬液,分别经小鼠尾静脉注射,建立各组小鼠感染模型,各组小鼠感染模型建立成功后,分别于第1、3、5、7、9、11、13、15天,进行各组小鼠肺泡灌洗,收集小鼠肺泡灌洗液,获取各组小鼠肺泡巨噬细胞。应用ELISA方法和Western blot技术检测各组小鼠肺泡巨噬细胞TfR和Fn的表达。利用ELISA方法检测各组小鼠肺泡巨噬细胞TfR的表达结果显示:H37RV组与BCG组小鼠肺泡巨噬细胞TfR表达均高于空白对照组,在感染第7、9、11天差异最明显,具有统计学意义(P<0.05)。利用Western blot技术检测各组小鼠肺泡巨噬细胞TfR表达结果显示:于模型建成后第7、9、11天,H37RN组、BCG组、空白对照组三组之间差异有统计学意义(P<0.05)。用ELISA方法和Western blot技术检测各组小鼠肺泡巨噬细胞内Fn表达结果显示:于模型建成后第7、9、11天,H37RV组与BCG组的小鼠肺泡巨噬细胞内Fn表达量明显减低,并且于第7天时表达量最第,异有统计学意义(P<0.05)。结核分枝杆菌感染小鼠,导致小鼠肺泡巨噬细胞TfR的表达增高,而Fn的表达降低。不同毒力的结核杆菌感染后Fn蛋白表达差异并不明显,而不同毒力的结核杆菌感染后TfR蛋白的表达有差异性。
To investigate the effects of different virulence of Mycobacterium tuberculosis infection on the expression of transferrin receptor (TfR) and ferritin (Fn) in mouse alveolar macrophages and their temporal changes. Inoculation of the prepared BCG vaccine and Mycobacterium tuberculosis H37Rv strain (hereinafter referred to as H37Rv strain) suspension into the tail vein of mice to establish the mouse model of infection, the mice in each group After the successful establishment of the infection model, alveolar lavage was performed on the 1st, 3rd, 5th, 7th, 9th, 11th, 13th and 15th days respectively, and the BALF was collected to obtain the alveolar macrophages Phagocytic cells. The expressions of TfR and Fn in alveolar macrophages were detected by ELISA and Western blot. The expression of TfR in alveolar macrophages of mice in each group was detected by ELISA. The results showed that the expression of TfR in alveolar macrophages of H37RV group and BCG group was higher than that of blank control group, and the difference was most obvious on the 7th, , With statistical significance (P <0.05). Western blot was used to detect the expression of TfR in alveolar macrophages in each group. The results showed that there was significant difference between the three groups in H37RN group, BCG group and blank control group on day 7, day 9 and day 11 <0.05). The expression of Fn in alveolar macrophages of mice in each group was detected by ELISA and Western blot. The results showed that the expression of Fn in alveolar macrophages of H37RV group and BCG group on the 7th, (P <0.05), and the expression level was the highest on the 7th day (P <0.05). Mycobacterium tuberculosis infection in mice, resulting in alveolar macrophage TfR mice increased expression of Fn decreased expression. The differences of Fn protein expression between different virulent strains of Mycobacterium tuberculosis were not significant, but the expression of TfR protein was different after the different virulence of Mycobacterium tuberculosis infection.