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目的鉴定动物实验中Wistar大鼠所感染寄生虫的种属和分析其感染特征。方法解剖检查正常条件下饲养的大鼠肝脏表面的囊泡,取囊中的虫体头节以10%甲醛及KOH溶液处理后光学显微镜下进行寄生虫的形态学鉴定;并对所感染部位的肝组织进行病理学检查。结果大鼠生长状况良好,无明显异常体征,寄生的虫体鉴定为带形囊尾蚴;本次所解剖检查的Wistar大鼠对该虫的感染率高达85.7%,感染部位均为肝脏,感染虫体数目为5～120个不等,可见带形囊尾蚴早期和成熟期两种形态;肝脏病理虫体周围有嗜酸性粒细胞、淋巴细胞和浆细胞浸润,未被虫体占位的肝小叶结构正常,未见肝脏纤维瘤样变。结论 Wistar大鼠在饲养中易反复感染带形囊尾蚴,并能耐受重度感染,作为动物模型,一方面应严防该虫的感染对实验结果的干扰,另一方面可作为一个很好的免疫应答的干预模型应用于其他疾病的研究。
Objective To identify the species of parasites infected by Wistar rats in animal experiments and analyze their infection characteristics. Methods Anatomical examination of the vesicles on the liver surface of rats fed under normal conditions was performed. Morphological identification of parasites was carried out under a light microscope with 10% formaldehyde and KOH solution. Liver tissue pathological examination. Results The rats grew well and had no obvious abnormal signs. The parasitic worms were identified as Cysticercus cellulosae. The Wistar rats dissected this time had an infection rate as high as 85.7% of the worms. The infected parts were both liver and infected worms Body number ranging from 5 to 120, showing the early and mature cysticercosis with two forms; liver pathological parasites eosinophils, lymphocytes and plasma cells infiltration, were not occupied by the body of the hepatic lobule The structure is normal, no liver fibroma-like changes. Conclusion Wistar rats are susceptible to repeated cysticercosis in feeding and can tolerate severe infections. As an animal model, the Wistar rats should be prevented from interfering with the experimental results on the one hand, and on the other hand, can be used as a good immune The intervention model of response should be applied to the study of other diseases.