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目的探讨肌醇六磷酸体外对人骨肉瘤U2OS细胞的影响及其机制。方法通过检测细胞增殖活性、超微扫描电镜观察、荧光染色法检测细胞凋亡及c-myc蛋白表达来研究肌醇六磷酸对U2OS细胞的影响。结果肌醇六磷酸能够抑制人骨肉瘤细胞U2OS的增殖,扫描电镜观察可见细胞表面超微结构发生凋亡改变,荧光显色证实肌醇六磷酸能够促细胞凋亡,c-myc蛋白的表达降低。结论肌醇六磷酸在体外对人骨肉瘤U2OS细胞增殖的抑制机制可能与诱导细胞凋亡及调控c-myc蛋白表达相关。
Objective To investigate the effect of phytate on human osteosarcoma U2OS cells and its mechanism. Methods The effects of phytate on U2OS cells were studied by measuring cell proliferation, scanning electron microscopy and fluorescent staining for the expression of c-myc. Results Phytate could inhibit the proliferation of human osteosarcoma cell line U2OS. The ultrastructure of cell surface was observed by scanning electron microscopy. Fluorescence staining confirmed that phytate could promote apoptosis and reduce the expression of c-myc protein. Conclusions The mechanism of inhibition of inositol phytate on the proliferation of human osteosarcoma U2OS cells may be related to the induction of apoptosis and the regulation of c-myc protein expression.