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目的:提取野生糙苏花中的挥发油,分析挥发油的组分,探讨其生物活性为开发这一资源提供理论依据。方法:用水蒸气蒸馏法从新鲜的糙苏花中提取挥发油,用气相色谱-质谱联用技术对挥发油组分进行分离和鉴定,运用气相色谱面积归一化法确定各组分的相对含量,对糙苏花的挥发油进行抗菌试验。结果:从糙苏花的挥发油中鉴定出55个组分,占全油的91.53%,糙苏花的挥发油有明显的抗菌活性。结论:糙苏花的挥发油中以单萜和倍半萜类物质居多,含量较高的组分是甲苯(17.28%)、邻苯二甲酸二异丁酯(13.38%)、α-里哪醇(12.38%)、二苯胺(7.17%)、1-辛烯-3-醇(6.22%)等,其挥发油对试验菌株有明显的抑制和灭活作用特别是对肺炎球菌32201株和肠炎沙门菌50040株表现的更为明显。
OBJECTIVE: To extract essential oils from wild Botrytis purpurea, analyze the components of essential oils, and explore their biological activities to provide a theoretical basis for the development of this resource. Methods: The essential oils were extracted from steam flowers by steam distillation. The components of volatile oils were separated and identified by gas chromatography-mass spectrometry. The relative contents of each component were determined by gas chromatography area normalization method. Sulphur volatile oil is used for antibacterial tests. RESULTS: Fifty-five components were identified from the volatile oil of S. frondosa, which accounted for 91.53% of the total oil. The volatile oil of S. frondosa had significant antibacterial activity. Conclusion: The volatile oil of S. frondosa is mainly monoterpenes and sesquiterpenoids. The higher content components are toluene (17.28%), diisobutyl phthalate (13.38%), α-linalool. (12.38%), diphenylamine (7.17%), 1-octen-3-ol (6.22%), etc. The volatile oil has obvious inhibitory and inactivating effects on the tested strains, especially for Streptococcus pneumoniae 32201 and Salmonella enteritidis. The 50040 strain showed more pronounced.