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目的 探索帕金森病 ( PD)发病过程中多巴胺能神经元可能的死亡方式。 方法 采用TUNEL法、流式细胞术及免疫组化方法检测 6-羟多巴胺 ( 6-OHDA)诱导 PD模型鼠中脑黑质神经细胞凋亡情况。 结果 TUNEL法及流式细胞术均观察到 PD模型鼠中脑黑质神经细胞凋亡样改变。在 2周、1个月、3个月模型鼠中 ,TUNEL法显示黑质神经细胞凋亡率分别为 ( 48.5± 5.5) %、( 1 1 .0± 3 .0 ) %、( 6.5± 3 .0 ) %,流式细胞术检测亚 G1期细胞百分率分别为 ( 57.3± 9.7) %、( 1 1 .7±3 .6) %、( 6.5± 1 .7) %,两种方法结果具有一致性。2周模型组与 1个月、3个月模型组比较 ,差异有显著性 ( P<0 .0 0 1 )。 结论 6-OHDA诱导 PD模型鼠中脑黑质神经元存在以细胞凋亡为主的死亡方式 ,细胞凋亡在 PD发病中可能起关键作用。
Objective To explore the possible ways of death of dopaminergic neurons during the pathogenesis of Parkinson’s disease (PD). Methods TUNEL, flow cytometry and immunohistochemistry were used to detect the apoptosis of substantia nigra neurons in 6-hydroxydopamine-induced PD model rats. Results TUNEL method and flow cytometry were observed in PD model mice brain neuronal apoptosis like changes. TUNEL assay showed that the apoptotic rate of substantia nigra neurons were (48.5 ± 5.5)%, (110 ± 3.0)%, (6.5 ± 3)% at 2 weeks, 1 month and 3 months respectively .0%). The percentages of cells in sub-G1 phase detected by flow cytometry were (57.3 ± 9.7)%, (11.7 ± 3.6)% and (6.5 ± 1.7)%, respectively. consistency. The difference between the two-week model group and the one-month and three-month model group was significant (P <0.01). Conclusions 6-OHDA induced neuronal apoptosis in the substantia nigra of PD model rats, which is the predominant death pattern. Apoptosis may play a key role in the pathogenesis of PD.