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背景 业已证实造血前体细胞可导致内皮细胞及造血干细胞(HPCs)增加,提示也许存在着公共的生长因子。研究设计与方法 研究血管内皮生长因子结合Tpo肽类作用剂(TPOA)、Flt-3L(F)、SCF(S)在长期培养起始细胞(LTC-IC)、CFU、分化、以及脐血(CB)CD34+细胞转导在4周的培养期间作用。结果 第4周,培养物内包含有T/F/S和VEGF,在10,000个细胞里以3.4的周期频率LTC-IC上升1,000倍(从37±8升到37,012±14,329)。而当培养物也含有T/F/S无VEGF时,在10,000个细胞里以1的周期频率LTC-IC上升
Background It has been demonstrated that hematopoietic precursor cells lead to an increase in endothelial cells and hematopoietic stem cells (HPCs), suggesting that there may be public growth factors. Research Design and MethodsTo investigate the effects of Flt-3L (F), SCF (S) on long-term culture of starting cells (LTC-IC), CFU, differentiation, and umbilical cord blood CB) CD34 + cell transduction during 4 weeks of culture. Results At week 4 cultures contained T / F / S and VEGF, which increased by 1,000-fold (from 37 ± 8 to 37,012 ± 14,329) in 10,000 cells at a 3.4 cycle frequency of LTC-IC. When cultures also contained T / F / S without VEGF, LTC-IC rose at a cycle frequency of 1 in 10,000 cells