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目的:比较耐力训练和间歇性速度训练对静息骨骼肌糖酵解能力及线粒体PDK4、CPT-1基因转录的影响;方法:30只大鼠随机分为3组:安静组(C,n=10)、耐力训练组(E,n=10)、间歇性速度训练组(S,n=10),训练8周。间歇性速度训练:每天9～10次10s极量强度(≥42m/min)的跑台运动,间歇时间30～60s;耐力训练:每天30～60min低强度(≤16.7m/min)的持续跑台运动;每周均训练6天。最后一次训练结束后的24～48h内切取腓肠肌,比色法检测丙酮酸、乳酸、HK、PK活性,Real-timePCR检测PDK4、CPT-1的mR-NA表达;结果:1)E组和S组丙酮酸均非常显著地高于C组(P<0.01),E组与S组无显著差异;乳酸浓度E组与C组无显著差异,但S组显著高于E组(P<0.05)和C组(P<0.01);2)E组(P<0.05)和S组(P<0.01)HK活性显著高于C组,但E组、S组PK活性与C组无显著差异;E组与S组的HK、PK活性均无显著差异;3)E组PDK4mRNA表达显著低于C组(P<0.05),S组CPT-1mRNA表达显著高于C组(P<0.05),E组与S组的PDK4、CPT-1mRNA表达均无显著差异;结论:1)耐力训练与间歇性速度训练都能提高静息骨骼肌的丙酮酸水平,但只有间歇性速度训练提高静息骨骼肌的乳酸水平,说明间歇性速度训练很可能使骨骼肌在静息时的无氧代谢已处于活跃状态。耐力训练使丙酮酸升高则可能是脂肪酸氧化能力提高所必需的匹配效应;2)耐力训练与间歇性速度训练都能提高HK活性,但对PK活性无影响。耐力训练与间歇性速度训练在糖脂代谢中有着许多类似效应。间歇性速度训练也能作为一种节省时间的方式提高有氧代谢能力,但在提高有氧代谢能力的同时也能促进静息骨骼肌丙酮酸向乳酸的转化;3)耐力训练使PDK4转录抑制,间歇性速度训练使CPT-1转录上调,这与各训练方式下静息骨骼肌的乳酸水平有着高度一致性。
Objective: To compare the effects of endurance training and intermittent speed training on resting skeletal muscle glycolysis and mitochondrial PDK4 and CPT-1 gene transcription.Methods: Thirty rats were randomly divided into three groups: Quiet group (C, n = 10), endurance training group (E, n = 10), intermittent speed training group (S, n = 10), training for 8 weeks. Intermittent speed training: 9 ~ 10 times per day 10s Extreme intensity (≥42m / min) treadmill, intermittent time 30 ~ 60s; Endurance training: 30 ~ 60min per day Low-intensity (≤16.7m / min) Taiwan exercise; training every 6 days. The gastrocnemius muscles were harvested 24 to 48 hours after the last training. Pyruvate, lactate, HK and PK activities were detected by colorimetric assay. The mR-NA expression of PDK4 and CPT-1 was detected by Real-time PCR. Results: 1) (P <0.01). There was no significant difference between group E and group S. Lactic acid concentration had no significant difference between group E and group C, but group S was significantly higher than group E (P <0.05) And group C (P <0.01); 2) The activity of HK in group E (P <0.05) and group S (P <0.01) were significantly higher than those in group C There was no significant difference in the activity of HK and PK between group S and S; 3) The expression of PDK4 mRNA in group E was significantly lower than that in group C (P <0.05); The expression of CPT-1 mRNA in group S was significantly higher than that in group C (P <0.05) There was no significant difference in the expressions of PDK4 and CPT-1mRNA between S group and S group.Conclusion: 1) Both endurance training and intermittent speed training can improve the level of pyruvate in resting skeletal muscle, but only intermittent speed training can improve resting skeletal muscle Lactic acid level, indicating that intermittent speed training is likely to make the anaerobic metabolism of skeletal muscle at rest has been active. Endurance training increased pyruvate may be necessary matching effect of fatty acid oxidation ability; 2) Endurance training and intermittent speed training can improve HK activity, but had no effect on PK activity. Endurance training and intermittent speed training have many similar effects in glycolipid metabolism. Intermittent speed training can also improve aerobic metabolism as a time-saving way, but it also promotes the conversion of pyruvate to lactate from resting skeletal muscle while improving aerobic metabolism; 3) Endurance training allows PDK4 transcriptional repression , And intermittent speed training up-regulated the transcription of CPT-1, which was highly consistent with the lactate level of resting skeletal muscle under each training mode.