在液氮低温保存红内期疟原虫的基础上,我们于1980年成功地建立“疟原虫子抱子全蚊低温保存法”。其后又利用鼠疟研究含红内期疟原虫肝组织低温保存法,获得了满意的结果。 材料与方法 1 疟原虫 系本所培养并经长期血传保种的伯氏疟原虫(Plasmodium berghei)抗氯喹株和正常株。 2 实验动物 昆明(KM)远交小鼠,由本所实 Based on the cryopreservation of red-phase Plasmodium at liquid nitrogen, we successfully established the “Plasmodium falciparum whole-mosquito cryopreservation method” in 1980. Later on, the murine malaria was used to study the cryopreservation of the liver tissues of Plasmodium falciparum with red blood cells, and satisfactory results were obtained. Materials and methods 1 Plasmodium berghei chloroquine resistant strains and normal strains cultivated by the malaria parasite and maintained by long-term blood donation. 2 experimental animals Kunming (KM) far-off mice, by the real