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为明确CO_2气调胁迫对药材甲能源物质的影响,采用不同CO_2浓度(10%、30%、50%、70%和90%)对其分别胁迫2、4、6、8 h后,测定其体内多糖、可溶性蛋白质及脂肪等能源物质的含量,并计算其利用率,探讨药材甲对气调胁迫的生理生化响应。结果表明,相较于对照,随胁迫时间的延长及CO_2浓度的升高,药材甲能源物质的含量均逐渐减少,利用率则逐渐增加。10%CO_2浓度胁迫2 h下,单头药材甲多糖、蛋白质及脂肪的含量均最高,分别为13.94、162.87和35.14μg;其利用率最低,分别为4.85%、2.04%和11.02%。90%CO_2浓度胁迫8 h下,其含量最低,分别为11.02、145.69和19.08μg;利用率最高,分别为24.80%、12.37%和51.68%。不同处理下,药材甲对3种能源物质的利用率均差异显著,为脂肪>多糖>可溶性蛋白质。表明CO_2浓度和胁迫时间是导致药材甲能源物质消耗的重要因素,而其对不同能源物质不同的利用率,则可能与昆虫抗气性的形成密切相关。
In order to clarify the effect of CO2-induced stress on the energy sources of Chinese herbs, the effects of CO2 concentration (10%, 30%, 50%, 70% and 90% Body polysaccharides, soluble protein and fat and other energy substances in the content, and calculate the utilization rate, to discuss the physiological and biochemical response of a drug-induced stress. The results showed that compared with the control, with the prolonging of stress time and the increase of CO 2 concentration, the contents of energy substance in A were decreased and the utilization rate was gradually increased. Under the condition of 10% CO 2 concentration for 2 h, the content of hypromellose, protein and fat were the highest, which were 13.94, 162.87 and 35.14 μg, respectively. The lowest utilization rates were 4.85%, 2.04% and 11.02% respectively. Under the 90% CO 2 concentration for 8 h, the content was the lowest, which were 11.02, 145.69 and 19.08 μg, respectively. The highest utilization rates were 24.80%, 12.37% and 51.68% respectively. Under different treatments, the utilization rate of three kinds of energy substances of medicinal material A was significantly different, which was fat> polysaccharide> soluble protein. The results showed that the CO 2 concentration and stress time were the important factors that led to the consumption of energy sources in the medicinal materials. The different utilization rates of different energy sources may be closely related to the formation of gas-resistant insects.