论文部分内容阅读
应用哈茨木霉 (Trichodemaharzianum )T88菌株和深绿木霉 (T .atroviride)T95菌株分别与杨树烂皮病菌 (Cytosporachrysosperma)和杨树水泡溃疡病菌 (Dothiorellagregaria)进行了平皿对峙培养和载片对峙培养 ;并研究了两种木霉菌产生的挥发性代谢物对病原菌菌落的影响和其非挥发性代谢物对菌丝干重的影响 ;分别在光学显微镜和扫描电镜下观察了两种木霉菌的重寄生现象。结果表明 :对峙培养中木霉菌T88和T95对两种病原菌均有明显的抑制作用。两种木霉菌均能产生挥发性代谢物质并不同程度地抑制病菌菌落的生长 ,在密闭条件下T95的抑制率最高达到 80 .30 %。两种木霉菌产生的非挥发性代谢物质可以强烈抑制病原菌的生长、明显降低其菌丝干重 ,并具有热稳定性。在光学显微镜和扫描电镜下 ,可以观察到木霉菌的菌丝在病原菌的菌丝上平行或波浪式生长 ,并在其上产生钩状分枝、吸器或附着胞吸附于病原菌的菌丝上 ,或穿透病原菌的菌丝生长 ,或使病原菌的菌丝细胞原生质浓缩和菌丝断裂
Trichoderma harzianum T88 strain and T. atroviride strain T95 were respectively used for plate confrontation culture and slide confrontation culture with Cytospora chrysosperma and Dothiorella gregaria The effects of volatile metabolites produced by the two Trichoderma species on the colonies of pathogenic bacteria and the effects of their non-volatile metabolites on the dry weight of mycelia were also studied. The effects of two kinds of Trichoderma reesei were observed under light microscope and scanning electron microscope Parasitic phenomenon. The results showed that Trichoderma T88 and T95 were significantly inhibited in both pathogenic bacteria. Both Trichoderma species could produce volatile metabolites and inhibit the growth of pathogenic bacteria to some extent. Under confined conditions, the highest inhibition rate of T95 was 80.3%. The two non-volatile metabolites produced by Trichoderma spp. Can strongly inhibit the growth of pathogenic bacteria, significantly reduce their mycelial dry weight and have thermal stability. Under the light microscope and scanning electron microscope, it is observed that the mycelium of Trichoderma sp. Grows parallelly or undulately on the mycelium of the pathogen and has hook-shaped branches, adsorbents or attachment cells adsorbed on the mycelium of the pathogen, Or penetrate pathogen mycelial growth, or pathogenic bacteria hyphal cell protoplasm concentration and mycelium break