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应用噻唑蓝 (MTT)法检测 O6-苄基鸟嘌呤(O6- BG)与 1 ,3-二 (2 -氯乙基 ) -亚硝基脲 (BCNU)合用的细胞毒作用及透射电镜检测凋亡细胞的方法研究了 O6- BG对 O6-烷基鸟嘌呤 - DNA烷基转移酶(O6- AGT )阳性的人肝癌细胞 SMMC- 772 1对BCNU细胞毒作用敏感性的影响及其与 BCNU合用治疗移植瘤的协同效果 .结果显示 :1 .5- 6.0 mg· L-1的 O6- BG预先作用 2 h后 ,SMMC- 772 1细胞对 BCNU的敏感性明显增加 ;0 .75- 6.0 mg· L-1的 O6- BG可完全快速地抑制肿瘤细胞的 AGT活性并持续 1 2 h;ip 90 mg· kg-1的 O6- BG预处理 2 h后给予 2 5mg·kg-1的 BCNU治疗 ,可使动物 sc接种的人肝癌移植瘤生长延迟 38.6d,诱导肿瘤细胞凋亡 ,并且可明显抑制肿瘤组织的转移酶活性 .说明 O6- BG与 BCNU合用于 AGT阳性的肿瘤将具有明显的治疗效果
Detection of cytotoxicity of O6-benzylguanine (O6-BG) combined with 1,3-bis(2-chloroethyl)-nitrosourea (BCNU) by thiazolyl blue (MTT) and transmission electron microscopy The method of cell death studied the effect of O6-BG on cytotoxicity of human liver cancer cell line SMMC-7721 positive for O6-alkylguanine-DNA alkyltransferase (O6-AGT) and its use in combination with BCNU. The synergistic effect of the treatment of xenograft tumors showed that the sensitivity of SMMC-7721 cells to BCNU was significantly increased after pretreatment with O.5-6.0 mg·L-1 O6-BG for 2 h; 0.75-6.0 mg· L-1 O6-BG completely inhibited the AGT activity of tumor cells completely and lasted for 12 h; EPO treatment with ip 90 mg·kg-1 O 2 - BG pretreated 2 h after administration of 25 mg·kg-1 BCNU. The growth of human hepatoma xenografts inoculated with animals can be delayed by 38.6 days, induce apoptosis of tumor cells, and significantly inhibit the activity of tumor metastases. O6-BG combined with BCNU for AGT-positive tumors will have significant therapeutic effect.