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目的:观察预防性应用滋阴清热复方中药对慢性移植物抗宿主病(cGVHD)模型小鼠的影响。方法:小剂量中药组、中剂量中药组、大剂量中药组受鼠从诱导起分别给予相应剂量的滋阴清热中药,阴性及阳性对照组给予等剂量生理盐水,诱导后第2、5、8、12周,检查骨髓细胞中的Y染色体数量,进行嵌合体分析。诱导后每3天观察受鼠的体重、毛发变化并评分。诱导后100天断颈处死小鼠,观察皮肤、肝脏、回盲端小肠的病理变化并评分。结果:阳性对照组和不同剂量中药干预组均可形成较为稳定的供受者混合嵌合体,给予滋阴清热中药预防后,大剂量中药组、中剂量中药组cGVHD的发病率降低,小鼠毛发改变、体重变化和肝脏、皮肤、肠道病理改变评分与阳性对照组比较,差异均有统计学意义,小剂量中药组上述指标与阳性对照组比较,差异均无统计学意义。结论:适当剂量的滋阴清热中药可预防单倍体相合移植后cGVHD的发生。
Objective: To observe the effect of prophylactic Ziyin Qingre Compound Chinese herbal medicine on chronic graft-versus-host disease (cGVHD) model mice. Methods: The rats in low-dose traditional Chinese medicine group, middle-dose traditional Chinese medicine group and high-dose traditional Chinese medicine group were given the corresponding dose of Ziyin Qingre TCM respectively. The negative and positive control groups were given equal doses of normal saline. After induction, , 12 weeks, the number of Y chromosomes in bone marrow cells was examined, and chimera analysis was performed. The weight and hair of the mice were observed and scored every 3 days after induction. Mice were sacrificed on day 100 after induction, pathological changes of skin, liver and ileocecal small intestine were observed and scored. Results: The positive control group and different doses of Chinese medicine intervention group can form relatively stable mixed chimerism donor. After giving Ziyin Qingre traditional Chinese medicine prevention, the incidence of cGVHD in large dose of traditional Chinese medicine group and middle dose of traditional Chinese medicine group decreased, Changes, body weight changes and liver, skin, intestinal pathological changes score compared with the positive control group, the differences were statistically significant, the small dose of traditional Chinese medicine group indicators compared with the positive control group, the difference was not statistically significant. Conclusion: The appropriate dose of Ziyin Qingre TCM could prevent the occurrence of cGVHD after haploidentical transplantation.