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目的分析ELISA,GICA和IHA(RIHA)3种方法在疫情处理中的特异性、敏感性及其在快速诊断方面的作用。方法对2起疫情的检测结果进行统计学分析。结果ELISA,GICA和IHA 3种方法检测鼠疫F1抗体的阳性率分别为32.9%,2.5%和10.9%,差异有统计学意义(χ~2=42.28,P<0.01)。结论在疫情处理过程中,同时使用ELISA,GICA和IHA方法检测血清中的鼠疫F1抗体,其结果快速、准确、可靠,既能快速判定疫情,又能及时发现感染者,对迅速判定疫情和有效控制疫情扩散均能起到很好作用。RIHA检验方法所需时间短、敏感性好、准确性高,是早期、快速的诊断方法。
Objective To analyze the specificity and sensitivity of three methods of ELISA, GICA and IHA (RIHA) in the treatment of epidemic situation and their roles in rapid diagnosis. Methods The detection results of two outbreaks were statistically analyzed. Results The positive rates of F1 antibody detected by ELISA, GICA and IHA were 32.9%, 2.5% and 10.9% respectively, with statistical significance (χ ~ 2 = 42.28, P <0.01). Conclusions During the treatment of the outbreak, the ELISA method for detecting F1 antibody in sera was also used. The results showed that the antibody was rapid, accurate and reliable. It could not only determine the epidemic quickly but also find out the infected person timely. Control of the spread of the epidemic can play a good role. RIHA test method requires a short time, good sensitivity, high accuracy, is an early, rapid diagnostic method.