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[目的]研究自组装阿霉素微囊对兔肝脏VX2移植瘤模型的治疗作用。[方法]18只新西兰大白兔,肝脏左中叶VX2肿瘤组织块接种,随机分为3组,每组6只。10d至肝脏肿瘤直径大于1cm后行药物肝动脉灌注,分别经肝动脉给予生理盐水(A组),阿霉素4mg/kg(B组),阿霉素微囊等效药物剂量4mg/kg(C组)。治疗后1d、3d、5d、7d、10d、14d分别PET/CT显像测定肿瘤体积、肿瘤生长率和最大标准化摄取值(SUVmax)的变化。14d显像结束后处死动物,常规病理HE染色观察。[结果]治疗1周后B组和C组肿瘤体积及生长率明显低于对照组A组(P<0.05),10d后C组肿瘤体积增长率及生长率最低。C组药物给予后肿瘤SUVmax值降低,与其它组差异有显著性(P<0.05),HE切片并可见到凋亡细胞增多。[结论]自组装阿霉素微囊可明显抑制兔肝脏VX2肿瘤糖代谢与生长,诱导肿瘤细胞凋亡,从而提高肝脏肿瘤化疗效果。
[Objective] To study the therapeutic effect of self-assembled doxorubicin microcapsules on VX2 xenograft model in rabbit liver. [Methods] Eighteen New Zealand white rabbits were inoculated into the left middle lobe of VX2 tumor tissue and divided into 3 groups randomly, 6 in each. The hepatic artery was perfused with hepatic artery more than 1 cm from 10 days to 10 days after hepatectomy. The rats were treated with normal saline (group A), adriamycin 4 mg / kg (group B) and doxorubicin microcapsules equivalent dose 4 mg / kg Group C). The changes of tumor volume, tumor growth rate and maximum normalized uptake value (SUVmax) were determined by PET / CT imaging at 1d, 3d, 5d, 7d, 10d and 14d after treatment. Animals were sacrificed on day 14 after imaging, and routine pathological HE staining was performed. [Results] After 1 week of treatment, the tumor volume and growth rate in group B and group C were significantly lower than those in control group A (P <0.05). After 10 days, the volume and growth rate of tumor in group C were the lowest. The tumor SUVmax value of group C decreased significantly compared with other groups (P <0.05), and the number of apoptotic cells increased in HE section. [Conclusion] Self-assembled doxorubicin microcapsules can significantly inhibit the glucose metabolism and growth of VX2 tumor in rabbit liver and induce the apoptosis of tumor cells, so as to improve the chemotherapy effect of liver tumors.