论文部分内容阅读
[目的]分析散发性结直肠癌中的hMLH1和hMSH2蛋白表达情况。[方法]选取经病理学确诊并在术前未接受过放疗或化疗的结直肠癌手术切除标本127例,以及内镜活检无肿瘤患者肠黏膜上皮20例。用免疫组化方法检测hMLH1和hMSH2蛋白表达情况。[结果]结直肠癌组织中hMSH2蛋白表达缺失率为65.4%(83/127),高于对照肠组织(20.0%,4/20)(χ2=14.714,P<0.001)。结直肠癌组织中hMSH2蛋白缺失率随T分期增加而增加(χ2=8.233,P=0.041);与N分期有关(χ2=20.235,P<0.001),有淋巴结转移者患者中hMSH2蛋白表达缺失率达87.1%(27/31),高于无淋巴结转移患者(54.2%)(χ2=9.250,P=0.002)。hMLH1蛋白表达缺失率为74.0%,与T分期(χ2=29.115,P<0.001)、N分期(χ2=9.807,P=0.006)、M分期(χ2=7.363,P=0.007)有关。[结论]结直肠癌组织中存在hMLH1和hMLH2蛋白表达缺失,通过免疫组化方法检测,可以简便、准确地发现错配修复基因的突变,可为后期的治疗和预后判断提供参考。
[Objective] To analyze the protein expression of hMLH1 and hMSH2 in sporadic colorectal cancer. [Method] 127 cases of colorectal cancer surgically diagnosed by pathology and without preoperative radiotherapy or chemotherapy and 20 cases of intestinal mucosa epithelium without endoscopic biopsy were selected. Immunohistochemistry was used to detect hMLH1 and hMSH2 protein expression. [Results] The loss rate of hMSH2 protein in colorectal cancer tissues was 65.4% (83/127), which was higher than that in control intestine (20.0%, 4/20) (χ2 = 14.714, P <0.001). The loss of hMSH2 protein in colorectal cancer tissues increased with the increase of T stage (χ2 = 8.233, P = 0.041). The correlation between the loss of hMSH2 protein expression in colorectal cancer tissues and the N stage (χ2 = 20.235, P <0.001) Up to 87.1% (27/31), higher than those without lymph node metastasis (54.2%) (χ2 = 9.250, P = 0.002). The loss of hMLH1 protein was 74.0%, which was related to T stage (χ2 = 29.115, P <0.001), N stage (χ2 = 9.807, P = 0.006) and M stage (χ2 = 7.363, P = 0.007) [Conclusion] The loss of hMLH1 and hMLH2 protein expression in colorectal cancer tissues can be detected by immunohistochemistry. The mutation of mismatch repair gene can be found easily and accurately, which may provide a reference for later treatment and prognosis.