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用多管微电极技术，在麻醉、自主呼吸的成年大鼠观察了微电泳谷氨酸（L-GLu）、γ-氨基丁酸（GABA）及其拮抗剂DL－2－氨基－5－磷酸戊酸（AP-5）、荷包牡丹碱（BIC）对前包氏复合体（pre－Botzingercomplex，preBotc）区神经元自发放电的影响，并测试了AP-5、BIC分别对L－Glu、GABA效应的阻断作用。结果显示，大多数被测试神经元（37／41个）被L－Glu兴奋，少数（4／41个）无反应：GABA使大多数被测试神经元（34／40个）抑制，对少数神经元（6／40个）无影响。AP-5对神经元放电无影响（19／34个）或产生抑制作用（15／34个）。BIC使神经元兴奋（10／30个）或无反应（20／30个）。L－Glu的兴奋作用和GABA的抑制作用呈量效依赖关系。9／21个受试神经元对L-Glu的兴奋反应被AP-5部分或完全阻断，大多数受试神经元（20／25个）对GABA的抑制反应被BIC部分或完全阻断。上述结果提示，成年大鼠pre－Botc区可能存在着内源性的Glu和GABA，它们分别通过NMDA受体和GABAA受体参与该区的神经信息传递过程。
Microdissection of glutamate (L-GLu), γ-aminobutyric acid (GABA) and its antagonist DL-2-amino-5-phosphate were observed in anesthetized and spontaneously breathing adult rats using multi- (AP-5) and bicuculline (BIC) on the spontaneous discharge of neurons in pre-Botzinger complex (preBotc), and the effects of AP-5 and BIC on the neuronal activity of L-Glu, GABA Effect of blocking effect. The results showed that most of the tested neurons (37/41) were excited by L-Glu and a few (4/41) did not respond: GABA inhibited most of the tested neurons (34/40) Yuan (6/40) no effect. AP-5 had no effect on neuronal discharge (19/34) or inhibited (15/34). BIC excited neurons (10/30) or no reaction (20/30). The excitatory effect of L-Glu and the inhibitory effect of GABA were dose-dependent. The excitatory response to L-Glu in 9/21 neurons was partially or completely blocked by AP-5. The inhibitory response to GABA in most of the neurons (20/25) was partly or completely blocked by BIC. The above results suggest that there may exist endogenous Glu and GABA in the pre-Botc region of adult rats, which participate in the neurotransmission process through NMDA receptor and GABAA receptor respectively.