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目的建立大鼠血浆中染料木素浓度的UPLC测定方法,探讨其在大鼠体内的药代动力学过程。方法色谱柱为Acquity UPLCTM BEH C18(100mm×2.1mm,1.7μm),流动相为乙腈-0.1%甲酸水(26:74),流速为0.4mL·min-1,检测波长为260nm,柱温40℃。采用UPLC法测定给药后大鼠血浆中染料木素浓度,利用DAS软件拟合并计算其药代动力学参数。结果血浆中染料木素在5～1000ng·mL-1范围内线性关系良好,提取回收率均大于90%,日间和日内精密度小于10%,符合生物样品分析要求。染料木素主要药动学参数Tmax、Cmax、t1/2、AUC(0→∞)分别为120min、106.7μg·L-1、306.59min、31543μg·L-1·min。结论该方法操作简便、快速、灵敏、专属性强,可用于染料木素大批量生物样本的定量分析及药代动力学研究。
Objective To establish a UPLC method for determining the concentration of genistein in rat plasma and investigate its pharmacokinetics in rats. Methods The chromatographic column was Acquity UPLCTM BEH C18 (100mm×2.1mm, 1.7μm), the mobile phase was acetonitrile-0.1% formic acid water (26:74), the flow rate was 0.4mL·min-1, the detection wavelength was 260nm, and the column temperature was 40. °C. UPLC was used to determine the concentration of genistein in the plasma after administration. The DAS software was used to fit and calculate the pharmacokinetic parameters. Results The linear relationship of genistein in plasma ranged from 5 to 1000 ng·mL-1. The recovery of extraction was more than 90%, and the intra- and intra-day precision was less than 10%, which was in accordance with the requirements of biological sample analysis. The main pharmacokinetic parameters of genistein Tmax, Cmax, t1/2, AUC (0→∞) were 120 min, 106.7 μg·L-1, 306.59 min, and 31543 μg·L-1·min, respectively. Conclusion The method is simple, rapid, sensitive and specific. It can be used for the quantitative analysis and pharmacokinetic study of genistein in large-volume biological samples.