以“凤凰白皮”洋葱种子为试材,采用转基因方法,将载体pBAC9075转入愈伤组织,研究花青素合成转录因子Bi和C1对转基因植株的影响。结果表明:经再生培养和草甘膦筛选,获得189棵再生植株,PCR检测发现5棵再生植株表现阳性,田间草甘膦筛选有7棵植株存活,其中2棵植株鳞茎有花青素积累,试验结果以期为花青素作为一种可视化跟踪系统用于洋葱转基因植株早期筛选提供依据。 Using “Phoenix white skin” onion seeds as test material, the vector pBAC9075 was transferred into callus by transgenic method to study the effects of anthocyanin biosynthesis transcription factors Bi and C1 on transgenic plants. The results showed that 189 regenerated plants were obtained through regeneration culture and glyphosate screening. Five regenerated plants were positive by PCR. Seven plants were selected by field glyphosate screening, of which two plants had anthocyanin accumulation, The experimental results provide a basis for anthocyanin as a visual tracking system for early screening of onion transgenic plants.