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[目的]研究RcLEA基因在月季品种中热诱导表达模式及RcLEA基因对多种非生物胁迫耐性。[方法]将耐热和不耐热月季品种进行38℃/3h热激处理,研究RCLEA基因在月季品种中的热诱导。表达模式;为验证月季RcLEA基因功能,将其转化E.coliBL21,将重组菌株BL21分别置于4℃、50℃及LiCl、NaCl、Na2CO3、CdCl2、H2O2胁迫下,研究重组菌株对高温、低温及非生物胁迫的响应。[结果]38℃/3h热激处理后,该基因在耐热月季品种‘曼海姆宫殿’(Schlossmannieim,SM)、‘赌城’(Lasvegas,LV)中强表达,而在不耐热品种‘新十全’(Kordes’Perfecta,KP)弱表达或不表达,表明该基因与月季耐热性关系密切。重组菌株提高了寄主大肠杆菌对高温、低温、重金属、高盐、高pH值、氧化等非生物胁迫的耐性,表明RcLEA参与了上述非生物胁迫的响应。[结论]该研究为后续该基因导入不耐热月季品种提高月季耐热品质及其机理研究提供了思路,也为月季等园林观赏植物的耐热品种筛选提供了理论支持。
[Objective] The research aimed to study the heat-induced expression pattern of RcLEA gene in rose varieties and the tolerance of RcLEA gene to various abiotic stresses. [Method] The heat-tolerant and heat-labile rose varieties were heat-shocked at 38 ℃ for 3 hours to study the heat-induced induction of RCLEA gene in rose varieties. In order to verify the function of RcLEA gene, the recombinant plasmid was transformed into E.coli BL21 and the recombinant strain BL21 was exposed to 4 ℃, 50 ℃ and LiCl, NaCl, Na2CO3, CdCl2 and H2O2 stress respectively. Abiotic stress response. [Result] After heat shock treatment at 38 ℃ for 3 hours, the gene was strongly expressed in the heat-tolerant Chinese rose varieties ’Schlossmannieim’ (SM) and Las Vegas (LV) Weakly expressed or not expressed Kordes’Perfecta (KP), indicating that the gene is closely related to the heat tolerance of roses. The recombinant strain increased the resistance of host E. coli to abiotic stresses such as high temperature, low temperature, heavy metals, high salt, high pH, and oxidation, indicating that RcLEA is involved in the abiotic stress response. [Conclusion] This study provided a new idea for the follow - up study on improving the heat tolerance and its mechanism of heat - tolerant rose varieties, and also provided theoretical support for the screening of heat - tolerant varieties of ornamental plants such as rose.