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检测了生长3年的转反义CCoAOMT基因杨树(Populus tremula×Populus alba)的制浆性能.该株系木质素含量比野生型对照下降约13%,G/S略有增加.化学成分分析表明,转基因杨树的灰分含量、冷水抽提物、热水抽提物、1%NaOH抽提物、综纤维素、戊聚糖及纤维素含量与野生型对照没有明显差别,但转基因杨树的苯醇抽提物含量略有增加.纤维特征分析表明,转基因杨树的纤维品质有所提高.实验室小规模制浆实验表明,降低木质素含量可以明显改善转基因杨树的制浆性能,表现为木质素更易于去除、纸浆得率增加和纤维聚合度提高.
The pulping performance of the antisense CCoAOMT gene poplar (Populus tremula × Populus alba) grown for 3 years was tested.The lignin content decreased by about 13% and the G / S slightly increased compared with the wild-type control.The chemical composition analysis The results showed that ash content, cold water extract, hot water extract, 1% NaOH extract, holocellulose, pentosan and cellulose content of transgenic poplars were not significantly different from the wild type control, but transgenic poplars The results of fiber characteristics showed that the fiber quality of transgenic poplar increased.Laboratory pulping experiments showed that reducing the content of lignin can significantly improve the pulping performance of transgenic poplar, The performance of lignin is easier to remove, increase the pulp yield and increase the degree of fiber polymerization.