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目的研究绿原酸和甘草酸联合应用体外抗呼吸道合胞病毒作用。方法以利巴韦林为阳性药,采用细胞病变抑制试验,噻唑蓝比色法检测细胞活性,观察绿原酸和甘草酸单用与合用不同比例(3∶1、2∶1、1∶1、1∶2)在Hep-2细胞中对呼吸道合胞病毒的抑制作用。结果在不同方式作用下,与绿原酸、甘草酸单独用药相比,联合组治疗指数增大,最大无毒浓度时病毒滴度小于单独用药组,抑制呼吸道合胞病毒的作用显著增强。当配比为绿原酸(133μg·ml~(-1))+甘草酸(67μg·ml~(-1))2∶1时,对呼吸道合胞病毒抑制率最大,治疗指数最高,病毒滴度最小。结论绿原酸与甘草酸联合用药对呼吸道合胞病毒的抑制具有协同增效作用,增强了体外抗呼吸道合胞病毒作用。
Objective To study the combination of chlorogenic acid and glycyrrhizin in vitro against respiratory syncytial virus. Methods Ribavirin was used as a positive drug. Cytopathic inhibitory assay was used. Cell viability was measured by thiazolyl blue colorimetric assay. Chlorogenic acid and glycyrrhizic acid were used in combination with different ratios (3:1,2:1,1:1 , 1: 2) inhibits respiratory syncytial virus in Hep-2 cells. Results Compared with chlorogenic acid and glycyrrhizin alone, the therapeutic index increased in the combination group and the virus titer in the largest non-toxic concentration group was less than that in the drug-alone group, and the effect of inhibiting respiratory syncytial virus was significantly enhanced. When the ratio of chlorogenic acid (133μg · ml -1) + glycyrrhizin (67μg · ml -1) 2: 1, the inhibition rate of respiratory syncytial virus was the highest, and the treatment index was the highest, Degree of the smallest. Conclusion The combined use of chlorogenic acid and glycyrrhizin has a synergistic effect on the inhibition of respiratory syncytial virus and enhances the anti-respiratory syncytial virus effect in vitro.