论文部分内容阅读
建立了沸水浴去血清蛋白质后用ＨＰＬＣ直接测定阿魏酸（ＦＡ）方法：方剂的血样预处理新方法分别用二维、三维ＨＰＬＣ和分光光度计定性测定ＦＡ后，用内标（香豆精）法定量。甲醇－乙酸－水（３８：０．３：６１．７）为流动相，Ｃ_(８)（ＯＤＳ_(２)）柱（１５０ｍｍ×４．６ｍｍ，５μｍ）为固定相时，ＦＡ的最低检测限 ６ ｎｇ；沸水浴 １０ ｍｉｎ的最低检测血清浓度是 ２０．２ ｎｇ／ｍＬ，线性范围 ３３． ７～２ １５６．８ ｎｇ／ｍＬ，ｒ＝０．９９９３；方法平均回收率（９３．５９±１．９３）％，日间及日内精密度ＲＳＤ值均为８．４４％。上法已用于人服川芎汤后经时变化的血清ＦＡ浓度测定。
A method was developed for the direct determination of ferulic acid (FA) by HPLC in a boiling water bath to remove serum proteins: a new method for the pretreatment of blood samples of formulas. After qualitative determination of FA by two-dimensional, three-dimensional HPLC and spectrophotometry, the internal standard (coumadin) was used. ) Quantitative method. When methanol-acetic acid-water (38:0.3:61.7) is the mobile phase, C_(8)(ODS_(2)) column (150mm×4.6mm, 5μm) is the lowest detection limit of FA 6 ng; The minimum serum concentration in the boiling water bath for 10 min was 20.2 ng/mL, with a linear range of 33. 7～2 156.8 ng/mL, r=0.9993; the average recovery of the method was (93.59±1.93)%, and the intraday and intraday precision RSD values were all 8.44%. The method has been used to determine the concentration of serum FA that changes with time after the human took Chuanxiong decoction.