论文部分内容阅读
目的:优选羌活-独活合提液中总香豆素的大孔吸附树脂纯化工艺条件。方法:采用UV测定总香豆素含量,检测波长300 nm。通过静态吸附-洗脱试验比较不同型号大孔树脂对总香豆素的分离纯化能力,以总香豆素含量为指标,利用单因素试验考察上样液质量浓度、上样量、洗脱剂体积分数及用量对羌活-独活合提液中总香豆素纯化工艺的影响。结果:D101型树脂对总香豆素具有较好的吸附和解吸附性能,最佳工艺条件为上样液质量浓度1.089 g·L-1,吸附流速1 BV·h-1,上样量2.5 BV,加4.5 BV水洗脱,弃去洗脱液,加80%乙醇2.5 BV洗脱,收集洗脱液;总香豆素吸附率、洗脱率及纯度分别为91.03%,84.80%,63.65%。结论:D101型树脂对羌活-独活合提液中总香豆素具有较好的分离纯化效果,优化的纯化工艺操作简单、稳定可行。
OBJECTIVE: To optimize the conditions of macroporous adsorption resin purification of total coumarins in Notopterygium - Proceed together. Methods: The total coumarin content was determined by UV detection wavelength 300 nm. Through the static adsorption-elution test, the separation and purification of total coumarins from different macroporous resins were compared. Using the total coumarin content as an index, the single factor test was used to investigate the effects of the mass concentration, Effect of Volume Fraction and Amount on Purification of Total Coumarins in Notopterygium - Proceeding Rejuvenation. Results: D101 resin had good adsorption and desorption properties for total coumarin. The optimum conditions were the mass concentration of sample 1.089 g · L-1, adsorption flow rate 1 BV · h-1, sample volume 2.5 BV , Add 4.5 BV water to elute, discard the eluent, add 80% ethanol 2.5 BV to elute and collect the eluent. The total coumarin adsorption rate, elution rate and purity are 91.03%, 84.80%, 63.65% . Conclusion: D101 resin has good separation and purification effects on total coumarins in Notopterygium - Proceed together. The optimized purification process is simple, stable and feasible.