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抗坏血酸(H_2A)是一个强还原剂,在人体内受微量过渡金属离子、其可溶性络合物及金属酶-抗坏血酸氧化酶的催化,经过两个单电子的转移,氧化成脱氢抗坏血酸(A).本文以Cu(Ⅱ)与酪氨酸组成的络合物为催化剂,测定抗坏血酸氧化过程的动力学参数及其可能存在的中间体,以便进一步了解H_2A在接近生理条件氧化时,电子转移的途径。实验部分本实验是在pH=7.44以纯氧饱和的磷酸缓冲溶液中进行。除Cu(Ⅱ)-酪氨酸按文献
Ascorbic acid (H 2 A) is a strong reducing agent that is oxidized to dehydroascorbic acid (A) by two single-electron transitions catalyzed by trace amounts of transition metal ions, their soluble complexes and the metalloenzyme-ascorbate oxidase in humans, In this paper, the complex of Cu (Ⅱ) and tyrosine was used as a catalyst to determine the kinetic parameters of ascorbic acid oxidation and its possible intermediates in order to further understand the pathway of electron transfer when H 2 A is oxidized near physiological conditions . Experimental Section This experiment was conducted in a phosphate buffer solution saturated with pure oxygen at pH = 7.44. In addition to Cu (Ⅱ) - tyrosine according to the literature