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[目的]研究苯并[α]芘(BaP)对人神经母细胞瘤SH-SY5Y细胞能量代谢的影响,进一步探讨BaP的神经毒性.[方法]不同浓度BaP染毒SH-SY5Y细胞24h,采用MTI法检测细胞活力;细胞丙二醛(MDA)测定试剂盒测定细胞内MDA含量;超氧化物歧化酶(SOD)分型测试盒测定细胞内锰(Mn)-SOD含量;Seahorse XFp细胞能量分析仪检测细胞耗氧率(OCR)及胞外酸化率(ECAR).[结果]随着BaP染毒浓度的增大,细胞活力明显下降(P趋势＜0.01);随着BaP染毒浓度的增大,细胞内MDA水平升高(P趋势＜O.01),Mn-SOD水平降低(P趋势＜0.01),1.0、10.0、100.0 μmol/LBaP组细胞内MDA含量明显高于对照组,Mn-SOD含量明显低于对照组(P＜0.05);随着BaP染毒浓度的增大,细胞的基础有氧呼吸、ATP偶联有氧呼吸速率、有氧呼吸最大值、糖酵解水平、糖酵解最大值及糖酵解储备值均呈下降趋势(P趋势＜0.01),各浓度组基础有氧呼吸、ATP偶联有氧呼吸速率明显低于对照组(P＜0.05);10.0、100.0 μmol/L BaP组有氧呼吸最大值、有氧呼吸储备值、糖酵解水平、糖酵解最大值及糖酵解储备值明显低于对照组(P＜0.05).不同浓度BaP处理组MTT光密度值及Mn-SOD含量与基础有氧呼吸、ATP偶联有氧呼吸速率、有氧呼吸最大值、有氧呼吸储备值、糖酵解水平、糖酵解最大值及糖酵解储备值均呈正相关(P＜0.05),细胞内MDA含量与各细胞生物能量参数均呈负相关(P＜0.05).[结论] BaP造成SH-SY5Y细胞氧化损伤,降低线粒体呼吸功能,较高浓度时损伤有氧呼吸储备能力及糖酵解功能.“,”[Objective] To study the effect of benzo[a]pyrene (BaP) on energy metabolism of human neuroblastoma SH-SY5Y cells,and further explore the potential neurotoxicity of BaP.[Methods] SH-SY5Y cells were exposed to different concentrations of BaP for 24 h.Cell viability was determined by MTI assay.Malondialdehyde (MDA) content was measured by MDA assay kit,manganese (Mn)-superoxide dismutase (SOD) content by SOD typing test kit,and cell oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) by Seahorse XFp cell energy analyzer.[Results] As the concentration of BaP increased,the cell viability decreased (Ptrend ＜ 0.01),the intracellular MDA level increased (Ptrend ＜ 0.01),and the Mn-SOD level decreased (Ptrend ＜ 0.01).The cells treated with 1.0,10.0,and 100.0 tmol/L BaP showed significantly higher MDA levels and significantly lower Mn-SOD levels than the control cells (P＜ 0.05).As the concentration of BaP increased,the basal OCR,ATP-linked OCR,maximal respiration,glycolysis,glycolytic capacity,and glycolytic reserve displayed a downward trend (Ptrend＜ 0.01),the basal OCR and ATP-linked OCR of the exposed groups were significantly lower than those of the control group (P ＜ 0.05),and the maximal respiration,spare respiratory capacity,glycolysis,glycolytic capacity,and glycolytic reserve of the 10.0 and 100.0 μmol/L BaP groups were significantly lower than those of the control group (P ＜ 0.05).Optical density of MTT and Mn-SOD level of the BaP exposed groups were positively correlated with basal OCR,ATP-linked OCR,maximal respiration,spare respiratory capacity,glycolysis,glycolytic capacity,and glycolytic reserve (P ＜ 0.05).Intracellular MDA level was negatively correlated with various cellular bioenergy indicators (P ＜ 0.05).[Conclusion] BaP can cause oxidative damage in SH-SY5Y cells,reduce mitochondrial respiratory function,and impair the spare respiratory capacity and glycolytic function of SI-I-SYSY cells at higher concentrations.