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Objective: To investigate the expression of fragilehistidine triad (FHIT) gene and its correlation withclinicopathological features and correlation with mismatchrepair protein (mainly MLH1 and MSH2) in humansporadic colorectal carcinoma (SCC). Methods:Immunohistochemistry SP method was used to determinethe expression of FHIT, MLH1 and MSH2 protein insurgically resected specimens of 84 human SCC. Results:The positive rates of FHIT, MLH1 and MSH2 proteinexpression were 48.81%, 92.86% and 100% respectively.Loss or reduced expression of FHIT protein was not related with tumors clinicopathological features such as age, gender, tumors site and histological type (P>0.05), but wascorrelated with tumors invade depth, degree of thedifferentiation, Ducks?stage and metastasis (P<0.05). There was no relationship between FHIT gene expression andMLH1 protein (r=0.0991, P>0.05) and MSH2 protein(r=0.0000, P=l.00) expression in human SCC. Conclusion:Absent or reduction of FHIT gene expression consists ofhigh proportion and is a frequent event in SCC. FHIT gene is involved in the development and progression of humanSCC and may be a candidate tumors suppressor gene. The relationship between alteration of FHIT gene expression and mismatch repair protein (mainly MLH1 and MSH2)deserved further study in human SCC.
Objective: To investigate the expression of fragilehistidine triad (FHIT) gene and its correlation with clinicopathological features and correlation with mismatchrepair protein (mainly MLH1 and MSH2) in humansporadic colorectal carcinoma (SCC). Methods: Immunohistochemistry SP method was used to determine the expression of FHIT, MLH1 and MSH2 protein insurgically resected specimens of 84 human SCC. Results: The positive rates of FHIT, MLH1 and MSH2 proteinexpression were 48.81%, 92.86% and 100% respectively. Loss or reduced expression of FHIT protein was not related with tumors clinicopathological features such There was no relationship between FHIT gene expression and MLH1 protein (r <0.05), but wascorrelated with tumors invade depth, degree of the differentiation, Ducks? stage and metastasis (P <0.05) = 0.0991, P> 0.05) and MSH2 protein (r = 0.0000, P = l.00) expression in human SCC. Conclusion: Absent or reduction of FHIT gene expression consists of o The relationship between alteration of FHIT gene expression and mismatch repair protein (mainly MLH1 and MSH2) deserved further study in human SCC.