论文部分内容阅读
探讨病毒与人类基因组同源性问题。选用ＥＢ病毒（ＥＢＶ），肠道病毒（ＥＶｓ）、单纯疱疹病毒－１型（ＨＳＶ－１），丙型肝炎病毒（ＨＣＶ）特异性引物，以６份健康成人外周血ＤＮＡ为模板，分别进行聚合酶链式反应（ＰＣＲ）。初步结果表明：有５份ＤＮＡ样品可通过ＥＢＶ、ＨＣＶ特异性引物扩增。ＥＢＶ引物扩增后的片段大小在１５４～２３４ｂｐ之间；ＨＣＶ引物扩增后的片段大小约２００ｂｐ和１２０ｂｐ。提示病毒某些基因可能存在人类基因组中或两者具有很高的同源性。
Explore the issue of homology between the virus and the human genome. The EBV, EVs, HSV-1 and HCV-specific primers were selected and 6 healthy adults peripheral blood DNA Polymerase chain reaction (PCR). The preliminary results show that: 5 copies of DNA samples can be amplified by EBV, HCV-specific primers. EBV primers amplified fragments between 154 ~ 234bp; HCV primers amplified fragment size of about 200bp and 120bp. Suggesting that some genes of the virus may exist in the human genome or both have very high homology.