本文介绍了一种检测肽是否吸附到聚苯乙烯板上的称为生物素-亲和素-生物素复合物(ABC)试验的简单化学方法,以助正确解释多肽抗体筛选中免疫吸附试验阴性的结果。具体程序是将合成的含11～27个氨基酸的肽(属人肌肉烟酸胆碱受体的α亚单位和人的小脑抗原等)加入板中,用NHS-LC-生 This article describes a simple chemical method called the biotin-avidin-biotin complex (ABC) assay that detects whether peptides are adsorbed onto a polystyrene plate to help correctly interpret the negative immunosorbent assay for peptide antibody screening the result of. The specific procedure is to add the synthesized peptide of 11 to 27 amino acids (α subunit of human muscle nicotinic acetylcholine receptor and human cerebellar antigen, etc.) to the plate, and then use NHS-LC-