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目的:对五年生人参叶生长发育过程中苹果酸脱氢酶(Malate Dehydrogenase MDH),乳酸脱氢酶(Lactate de-hydrogenase LDH),乙醇脱氢酶(Alcohol dehydrogenase ADH),葡萄糖-6-磷酸脱氢酶(Glucose-6-phosphate Dehydro-genase G6PDH),α-磷酸甘油脱氢酶(α-Glycerophosphate dehydrogenaseα-GPD)5种氧化还原酶的活力进行比较,了解人参叶生长情况。方法:分别于展叶期、开花期、绿果期、红果期、枯萎期采取人参叶样品。采用中性缓冲液提取粗酶液,应用分光光度法测定MDH、LDH、ADH、G6PDH、α-GPD的活力。结果:在人参叶生长发育过程中,MDH、ADH和LDH活力在展叶期明显高于其他时期;LDH、G6PDH在展叶期和结果期末期活力最高;α-GPD在结果期出现活力峰值。结论:通过测量MDH、LDH、ADH、G6PDH、α-GPD的活力变化可判断人参生长情况。
Objective: To investigate the effects of malate dehydrogenase (MDH), lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH), glucose-6-phosphate The activities of five oxidoreductases, Glucose-6-phosphate dehydrogenase (G6PDH) and α-Glycerophosphate dehydrogenase (GPD), were compared to understand the leaf growth of ginseng. Methods: Ginseng leaf samples were taken at leaf-setting stage, flowering stage, green-fruit stage, red-fruit stage and wilting stage respectively. The crude enzyme solution was extracted by neutral buffer, and the activity of MDH, LDH, ADH, G6PDH and α-GPD were determined by spectrophotometry. Results: During the growth and development of ginseng leaves, the activities of MDH, ADH and LDH in leaves were significantly higher than those in other periods. The activities of LDH and G6PDH were the highest at the leaf-setting stage and the end of the results period. Conclusion: The growth of ginseng can be judged by measuring the changes of vitality of MDH, LDH, ADH, G6PDH and α-GPD.