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肿瘤细胞对化疗药物产生抗药性是化疗失败的重要因素之一,业已旺实,决定多药耐药性的基因是肿瘤细胞抗药性(MDR)基因,分子量170kD是一种浆膜大分子糖蛋白,具有ATP依赖性主动转运泵将药物由细胞内泵出的功能。本实验采用经基因转移技术将人MDR_1 cDNA转染体外EJ膀胱癌细胞所获得的EJ膀胱癌耐药细胞,以及EJ膀胱癌细胞为研究对象。异搏定作为耐药调节剂逆转MDR_1
The resistance of tumor cells to chemotherapeutic drugs is one of the important factors for chemotherapy failure. It is already very promising. The gene that determines multidrug resistance is the tumor cell resistance (MDR) gene, and the molecular weight of 170 kD is a serosa macromolecular glycoprotein. The function of an ATP-dependent active transport pump that pumps the drug out of the cell. In this study, EJ bladder cancer cell lines obtained by transfecting human MDR1 cDNA into EJ bladder cancer cells by gene transfer technology and EJ bladder cancer cells were studied. Verapamil reverses MDR_1 as a drug resistance regulator