Plant homeobox domain(PHD)-type transcription factors(TFs) are involved in a variety of biological processes. However, its involvement in commercially important fruit ripening process remains largely unclear. In the present work, the characterization of a PHD-type TF termed MaPHD1 from banana fruit is reported. Multiple alignments of the deduced amino acid sequence revealed that Ma PHD1 showed a high homology with Arabidopsis thaliana Alfin1-like proteins belonging to plant-specific sub-family of PHD finger proteins. MaPHD1 was found localized in the nucleus and exhibited trans-repression ability. It was down-regulated by ethylene and ripening. Electrophoretic Mobility Shift Assay(EMSA) and transient expression analysis demonstrated that Ma PHD1 directly bound to the G-rich motifs in the promoter of Ma XTH6, which is associated with cell wall degradation, and subsequently repressed its expression. These findings suggest that MaPHD1 may be negatively associated with banana fruit ripening, at least in part, by the direct suppression of Ma XTH6. Taken together, these findings provide new insights into the transcriptional regulatory networks of banana fruit ripening. However, its involvement in a variety of biological processes. In the present work, the characterization of a PHD-type TF termed MaPHD1 from banana fruit is reported. Multiple alignments of the deduced amino acid sequence revealed that Ma PHD1 showed a high homology with Arabidopsis thaliana Alfin1-like proteins belonging to plant-specific sub-family of PHD finger proteins. It was down-regulated by ethylene and ripening. Electrophoretic Mobility Shift Assay (EMSA) and transient expression analysis of the PHD1 directly bound to the G-rich motifs in the promoter of Ma XTH6, which is associated with cell wall degradation, and subsequently repressed its expression. These findings suggest that MaPHD1 may be negatively associated with banana fruit ripenin g, at least in part, by the direct suppression of Ma XTH6. Taken together, these findings provide new insights into the transcriptional regulatory networks of banana fruit ripening.