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取培养30～35天的浙贝愈伤组织为冷冻材料,通过含5％DMSO(二甲基亚砜)的培养基预培养7天种10％DMSO+5％甘油的冷冻保护剂处理,能显著提高冷冻后愈伤组织的存活率。而且分步冷冻比快速冷冻效果更好。经过冷冻后的愈伤组织成功地得到增殖和再生鳞茎。
The calli of Zhebei shellfish cultured for 30 to 35 days were frozen and treated with a cryoprotectant containing 10% DMSO+5% glycerol pre-cultured for 7 days in a medium containing 5% DMSO (dimethyl sulfoxide). Significantly increase the survival rate of frozen callus. And step-by-step freezing is better than quick freezing. The frozen calli were successfully harvested and regenerated bulbs.