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目的通过二维超声及免疫组化方法观察肝动脉As2O3碘油栓塞对兔肝移植瘤生长、微血管密度(MVD)及血管内皮生长因子(VEGF)表达的影响。方法40只家兔肝内肿瘤种植后2周,随机分为5组,经肝动脉插管分别给予不同处理,实验设生理盐水灌注组(A组)、As2O3灌注组(B组)、单纯碘化油栓塞组(C组)、阿霉素+碘化油栓塞(D组)及As2O3+碘化油栓塞组(E组),As2O3的用量为2mg/kg。治疗后1周,采用二维超声检测肿瘤大小,计算肿瘤的生长率,病理观察肿瘤的坏死率,免疫组化方法测定瘤区的MVD及VEGF表达强度。结果治疗后1周,各组肿瘤体积分别为(1.441±0.26)cm3、(1.372±0.28)cm3、(0.578±0.16)cm3、(0.523±0.12)cm3和(0.508±0.10)cm3,各栓塞治疗组,肿瘤生长受到明显抑制;单纯碘化油栓塞及阿霉素+碘化油栓塞后,残余肿瘤区的MVD略有升高,两者分别为23.4±4.7和22.3±5.3,与阴性对照A组MVD为21.8±6.3相比,统计学无显著性意义;两组的VEGF表达强度分别为0.163±0.019和0.160±0.016,高于阴性对照A组(Ρ<0.05),A组的VEGF表达强度为0.140±0.02;As2O3+碘油栓塞组残余瘤区的MVD减低,VEGF表达减弱,两者分别为15.1±3.2和0.102±0.02。MVD与VEGF表达强度之间存在正相关。As2O3+碘油栓塞组肿瘤坏死率大于单纯碘油栓塞及阿霉素+碘油栓塞组
Objective To observe the effects of hepatic arterial As2O3 lipiodol embolization on the growth, microvessel density (MVD) and the expression of vascular endothelial growth factor (VEGF) in liver xenografts in rabbits by two-dimensional ultrasound and immunohistochemistry. Methods Forty rabbits with intrahepatic tumor were randomly divided into 5 groups after 2 weeks of implantation. The hepatic artery was cannulated to give different treatment. The experimental group was divided into three groups: normal saline group (group A), As2O3 group (group B) (Group C), doxorubicin + iodized oil embolism (group D), and As2O3 + iodized oil embolization group (group E). The dosage of As2O3 was 2mg / kg. One week after treatment, the size of the tumor was detected by two-dimensional ultrasound, the growth rate of the tumor was calculated, the necrosis rate of the tumor was observed by pathology, and the expression of MVD and VEGF were detected by immunohistochemistry. Results After 1 week of treatment, the tumor volume in each group was (1.441 ± 0.26) cm3, (1.372 ± 0.28) cm3, (0.578 ± 0.16) cm3, (0.523 ± 0.12) cm3 and (0.508 ± 0.10) cm3, Group, tumor growth was significantly inhibited; simple iodinated oil embolism and doxorubicin + iodized oil embolization, residual tumor area MVD increased slightly, both were 23.4 ± 4.7 and 22.3 ± 5.3, respectively, and negative control A The MVD of group A was significantly lower than that of 21.8 ± 6.3 (P <0.05). The VEGF expression intensity of the two groups were 0.163 ± 0.019 and 0.160 ± 0.016 respectively, which was higher than that of negative control group A (P <0.05) Was 0.140 ± 0.02. MVD was decreased and VEGF expression was decreased in the residual tumor area of As2O3 + lipiodol embolization group, which were 15.1 ± 3.2 and 0.102 ± 0.02, respectively. There is a positive correlation between MVD and VEGF expression intensity. As2O3 + lipiodol embolization group tumor necrosis rate was higher than simple lipiodol embolism and doxorubicin + lipiodol embolization group