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目的研究对碳青霉烯类抗生素耐药的黏质沙雷菌的基因分型。方法收集咸阳地区2010年-2014年15株对亚胺培南和美罗培南耐药的黏质沙雷菌株,应用PCR扩增和DNA序列分析,确认引起黏质沙雷菌耐碳青霉烯酶的基因型;将黏质沙雷菌与大肠埃希菌进行结合,并测定结合后大肠埃希菌对碳青霉烯类药物的MIC值。结果 PCR检测结果 9株(9/15)携带KPC-2基因,1株(1/15)携带IMP基因,1株(1/15)携带VIM基因,接合实验使大肠埃希菌与黏质沙雷菌有相似的耐药谱。结论耐碳青霉烯酶的黏质沙雷菌的主要耐药基因为KPC-2、IMP-2、VIM,是引起黏质沙雷菌耐药的主要原因。接合实验阳性提示耐药基因通过接合转录的方式在不同种属细菌间进行传播,应加强院内感染的监测和预防。
Objective To study the genotyping of carbapenem-resistant Serratia marcescens. Methods 15 strains of mucilaginous drug resistant to imipenem and meropenem from 2010 to 2014 in Xianyang were collected and confirmed by PCR amplification and DNA sequence analysis to confirm their resistance to carbapenemase Genotypes; Serratia marcescens and Escherichia coli combined, and determine the MIC value of the combined Escherichia coli on carbapenems. Results Nine of 9 (9/15) PCR products were carrying KPC-2 gene, 1 (1/15) carrying IMP gene and 1 (1/15) carrying VIM gene. The conjugation experiments between E. coli and mucilaginous Lei bacteria have similar resistance spectrum. Conclusion The main resistance genes of Serratia marcescens resistant to carbapenema are KPC-2, IMP-2 and VIM, which are the main causes of drug resistance of Serratia marcescens. The positive results of the conjugation experiments suggest that drug resistance genes are transmitted between different species of bacteria by conjugation and transcription, and the surveillance and prevention of nosocomial infections should be strengthened.