论文部分内容阅读
目的:明确单核细胞趋化蛋白-3(MCP-3)在喉鳞状细胞癌及癌旁正常组织的表达及分布,并探讨其在喉癌发病机制中的作用及临床意义。方法:采用RT-PCR,Westernblot及免疫组织化学技术检测MCP-3在喉癌组织和癌旁正常组织中的表达和分布。结果:RT-PCR检测表明,MCP-3mRNA在喉癌及喉正常组织内均有表达,MCP-3在喉癌组织中的表达较癌旁正常组织表达明显增多(P<0.05),并且在喉癌组织Ⅰ～Ⅱ期与Ⅲ～Ⅳ期表达差异有统计学意义(P<0.05);蛋白免疫印迹检测发现,喉癌组织和癌旁正常组织内均有MCP-3表达,MCP-3的表达位置在相对分子质量11000;喉癌组织中MCP-3的表达明显较癌旁正常组织的表达增多(P<0.01),并且在喉癌组织Ⅰ～Ⅱ期与Ⅲ～Ⅳ期表达之间差异有统计学意义(P<0.05);免疫组织化学染色发现:MCP-3阳性表达为黄色,主要分布在肿瘤细胞细胞质内,在喉正常的腺上皮组织中也可检测到阳性表达。结论:MCP-3可能与喉癌的发病机制有关,可能与肿瘤相关巨噬细胞的趋化和激活有关,肿瘤内的炎性微环境对肿瘤的发生、发展起重要作用。
OBJECTIVE: To determine the expression and distribution of monocyte chemoattractant protein-3 (MCP-3) in laryngeal squamous cell carcinoma and adjacent normal tissues, and to explore its role in the pathogenesis of laryngeal cancer and its clinical significance. Methods: The expression and distribution of MCP-3 in laryngeal carcinoma and adjacent normal tissues were detected by RT-PCR, Western blot and immunohistochemistry. Results: The expression of MCP-3 mRNA in laryngeal carcinoma and laryngeal tissues was significantly higher than that in normal laryngeal tissues (P <0.05). The expression of MCP-3 mRNA in laryngeal carcinoma tissues was significantly higher than that in adjacent normal tissues The expression of MCP-3 and the expression of MCP-3 in laryngeal carcinoma and adjacent normal tissues were detected by Western blot analysis. The expression of MCP-3 in laryngeal carcinoma tissues was significantly higher than that in adjacent normal tissues (P <0.01), and there was a significant difference between the expression of stage Ⅰ-Ⅱ and stage Ⅲ-Ⅳ Statistical significance (P <0.05). Immunohistochemical staining revealed that the positive expression of MCP-3 was yellow and mainly distributed in the cytoplasm of tumor cells. Positive expression of MCP-3 was also detected in normal laryngeal epithelium. CONCLUSION: MCP-3 may be related to the pathogenesis of laryngeal carcinoma, which may be related to the chemotaxis and activation of tumor-associated macrophages. The inflammatory microenvironment in the tumor plays an important role in tumorigenesis and development.