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[目的 ]检测血吸虫病患者的循环抗原。 [方法 ]用多抗与单抗夹心 EL ISA法检测日本血吸虫病患者血清循环抗原。 [结果 ]用于检测 15 0例血吸虫病患者的循环抗原 ,其敏感性平均为 84.7% (72 .0 %～ 91.0 % ) ,40例正常人未出现假阳性反应 ,74例其他寄生虫感染者中 ,有 2例并殖吸虫病患者阳性 ,其余均未出现交叉反应。 [结论 ]多抗与单抗夹心 EL ISA法是一种较为理想的循环抗原检测方法。
[Objective] To detect circulating antigens in schistosomiasis patients. [Method] Serum circulating antigens were detected in patients with schistosomiasis japonica by sandwich ELISA ELISAs. [Results] The circulating antigens used in detecting 150 schistosomiasis patients had an average of 84.7% (72.0% -91.0%) of the circulating antigens. There were no false positives in 40 normal controls and 74 other parasitic infections In 2 cases of paragonimiasis positive, the rest were not cross-reaction. [Conclusion] Multi-antibody and monoclonal antibody sandwich ELISA method is an ideal method for detecting circulating antigens.