The adsorption characteristics of inosine from fermentation solution on anion exchange resin under the condition of different PH, resin type are investigated. Besides 3 the desorption conditions are studied under different temperature.The adsorption and desorption mechanism are described to obtain the optimumtechnological condition of inosine extraction. The adsorption characteristics of in anion exchange resin under the different conditions of different resin types are investigated. In addition, three different conditions are studied under different temperatures. The adsorption and desorption mechanism are described to obtain the optimum technical condition of inosine extraction .