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目的观察星形胶质细胞上调基因1(AEG-1)在子宫颈癌组织中的表达及其与微血管密度、血管内皮生长因子(VEGF)和核因子κB(NF-κB p65)的相关性。方法收集温州医科大学附属第一医院手术切除的45例浸润性子宫颈癌标本和12例慢性子宫颈炎标本作为研究对象。采用免疫组化Max Vision法检测AEG-1,NF-κB p65和VEGF蛋白的表达水平,采用肿瘤微血管内皮标志物(CD34)结合Weidner计数法检测子宫颈癌组织新生的微血管密度(MVD)。在光学显微镜下观察AEG-1、NF-κB p65和VEGF在子宫颈癌组织中的阳性表达以及染色情况。采用Pearson相关性分析子宫颈癌组织中AEG-1表达与NF-κB p65、VEGF表达的相关性。结果 AEG-1在子宫颈癌和慢性子宫颈炎的表达差异有统计学意义(P<0.01),其表达水平与子宫颈癌患者发生脉管浸润和淋巴结转移有关(P<0.01),与患者年龄、分化程度、肿瘤大小、病理分型、宫旁浸润等因素无关(P>0.05)。经Pearson相关性分析发现,子宫颈癌组织中AEG-1表达与NF-κB p65(r=0.501,P<0.001)、VEGF(r=0.718,P<0.001)和MVD(r=0.815,P<0.001)呈正相关。结论 AEG-1在子宫颈癌组织中高表达,可促进子宫颈癌的微血管生成,其机制可能与AEG-1激活NF-κB信号途径进而上调VEGF水平有关。
Objective To investigate the expression of astrocyte up-regulated gene 1 (AEG-1) in cervical cancer and its relationship with microvessel density, vascular endothelial growth factor (VEGF) and nuclear factor κB (NF-κB p65) Methods 45 cases of invasive cervical cancer and 12 cases of chronic cervicitis were collected from the First Affiliated Hospital of Wenzhou Medical University. The expression of AEG-1, NF-κB p65 and VEGF were detected by immunohistochemistry Max Vision method. The neovascular microvessel density (MVD) of cervical cancer was detected by CD34 and Weidner counting. The positive expression and staining of AEG-1, NF-κB p65 and VEGF in cervical cancer tissues were observed under a light microscope. Pearson correlation analysis of cervical cancer AEG-1 expression and NF-κB p65, VEGF expression correlation. Results The expression of AEG-1 in cervical cancer and chronic cervicitis was significantly different (P <0.01). The expression level of AEG-1 was correlated with vascular invasion and lymph node metastasis in cervical cancer (P <0.01) , Differentiation degree, tumor size, histopathological type, parametrial infiltration and other factors (P> 0.05). The Pearson correlation analysis showed that the expression of AEG-1 in cervical cancer was positively correlated with the expression of NF-κB p65 (r = 0.501, P <0.001), VEGF (r = 0.718, 0.001) was positively correlated. Conclusion AEG-1 is overexpressed in cervical cancer and may promote the angiogenesis of cervical cancer. The mechanism may be related to the activation of NF-κB signal pathway by AEG-1 and the up-regulation of VEGF.