论文部分内容阅读
为观察重组日本血吸虫副肌球蛋白 (r Sj97)对水牛的保护效果。 2 0头水牛随机分为佐剂 (Quil A)对照组和抗原 (r Sj97)免疫组。对照组仅注射 Quil A,而免疫组注射 Quil A加 r Sj97,在第 0、2、15周肌肉注射。第 3次免疫后两周经腹部贴片攻击感染 10 0 0条日本血吸虫尾蚴 ,感染后第 49d剖杀冲虫 ,计数虫数和肝卵数。结果显示 ,与对照组相比 ,r Sj97免疫组的减虫率为 49.9% (P<0 .0 5 ) ,肝脏减卵率 5 7.3% (P<0 .0 1)。提示 r Sj97可诱导水牛产生一定程度的抗血吸虫攻击感染的保护性免疫力 ,并具有一定程度的抗病作用 ,进一步证实了副肌球蛋白可作为抗血吸虫病的候选疫苗分子。
To observe the protective effect of the recombinant Schistosoma japonicum paramyosin (r Sj97) on buffaloes. Twenty buffalo were randomly divided into four groups: control group (Quil A) and antigen group (r Sj97). In the control group, only Quil A was injected, while in the immunized group, Quil A plus r Sj97 was injected and intramuscularly at weeks 0, 2 and 15. Two hundred weeks after the third immunization, 100 0 S. japonicum cercariae were challenged by abdominal patch attack. The swine worms were dissected on the 49th day after infection, and the number of worms and the number of liver eggs were counted. The results showed that compared with the control group, the worm reduction rate of rSj97 immunized group was 49.9% (P <0.05), and that of the liver was 5 7.3% (P <0.01). It suggested that rSj97 could induce buffalo to produce a certain level of protective immunity against schistosomiasis infection and have a certain degree of resistance to disease, further confirming that paramyosin can be used as a vaccine candidate for anti-schistosomiasis.