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目的:研究白假丝酵母菌生物膜产生滞留菌的动态特点,为揭示其产生机制及相关途径奠定基础。方法:分别以两相型白假丝酵母菌标准菌液构建体外生物膜模型,CFU计数法统计不同时间段生物膜加药前真菌细胞繁殖数目及加药后滞留菌产生数目,采用SPSS11.5软件包对数据进行统计学分析;结合激光共聚焦显微镜(CLSM),观察生物膜的形态变化。结果:两相型菌液形成的不同时间段生物膜,真菌细胞繁殖数目及滞留菌数目均无显著差异。其中,真菌细胞繁殖数目呈“S”形生长,12 h后渐稳定;滞留菌0.5 h即大量产生,2 h后数目基本稳定,此时镜下生物膜处于微菌落始形成期。结论:白假丝酵母菌滞留菌的形成与其生物膜形成初期(2 h内)附着表面的诱导密切相关,而与生物膜成熟程度及两相型状态无显著关联。
OBJECTIVE: To study the dynamic characteristics of stagnant bacteria produced by the biofilm of Candida albicans and lay a foundation for revealing its mechanism of production and related pathways. Methods: In vitro biofilm model was established with two-phase Candida albicans standard bacteria respectively. The number of fungal cells multiplied by biofilm before drug addition and the number of resident bacteria after dosing were counted by CFU counting method. The data were statistically analyzed by software package. The morphological changes of biofilm were observed by confocal laser scanning microscopy (CLSM). Results: There was no significant difference in the numbers of biofilm and fungal cell multiplication and the number of bacteria retained in the biofilm at different time points. Among them, the number of fungal cell proliferation was “S” shape growth, gradually stabilized after 12 h; 0.5 h bacteria that is produced in large quantities, the number of 2 h after the basic stability, this time in microscopy microscopy biofilm in the formation phase. Conclusion: The formation of Candida albicans was closely related to the induction of the attachment surface during the initial biofilm formation (within 2 h), but not to the degree of biofilm maturation and the two-phase state.