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目的:探讨重楼总皂苷(RPTS)对多发骨折-脂多糖两次打击模型大鼠急性肺损伤(ALI)的影响。方法:将36只Wistar大鼠随机分成3组,除空白对照组外,其余2组按多发骨折-脂多糖两次打击急性肺损伤动物模型标准制模,分成重楼干预组和模型对照组,制模后1h,重楼干预组予重楼总皂苷(RPTS)灌胃。干预后6h,采血以ELISA法检测血清中TNF-α、IL-1β及IL-6浓度;各组留取肺组织,光镜下进行病理形态观察。结果:重楼干预组大鼠血清TNF-α、IL-1β及IL-6浓度显著低于模型对照组(P<0.001)。模型对照组,光镜下肺组织病理形态观察显示明显肺损伤;而重楼干预组肺损伤表现明显轻于模型对照组。结论:重楼总皂苷对多发骨折-脂多糖两次打击模型大鼠急性肺损伤具有保护作用,机制可能与下调血清中的TNF-α、IL-1β、IL-6水平从而减轻炎症反应有关。
Objective: To investigate the effect of RPTS on acute lung injury (ALI) in multiple-fracture-lipopolysaccharide two-hit rats. METHODS: Thirty-six Wistar rats were randomly divided into 3 groups. Except for the blank control group, the other 2 groups were divided into two groups according to the criterion of multiple fracture-lipopolysaccharides twice to hit the animal model of acute lung injury, divided into heavy floor intervention group and model control group. One hour after making the model, the replantation intervention group received intragastric administration of total saponins (RPTS). Six hours after the intervention, blood samples were taken for the detection of TNF-α, IL-1β and IL-6 concentrations in serum by ELISA. Lung tissues were obtained from each group and pathological observations were performed under light microscope. Results: The concentration of TNF-α, IL-1β and IL-6 in serum of rats in the re-building intervention group was significantly lower than that in the model control group (P<0.001). In the model control group, lung pathological observations under the light microscope showed significant lung injury, while those in the heavy floor intervention group were significantly lighter than the model control group. Conclusion: Total saponins of Rhizoma Paridis have protective effect on acute lung injury in multiple-fracture-lipopolysaccharide two-hit model rats, and the mechanism may be related to the down-regulation of TNF-α, IL-1β, and IL-6 levels in the serum to reduce the inflammatory response.