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为了探讨不同品种板栗对栗疫菌Cp-毒素胁迫响应的差异,应用叶圆片法,研究板栗不同抗性品种叶片经栗疫菌毒素Cp-处理后对其过氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)等防御酶活性的影响。结果显示:Cp-毒素浓度低于100μg/mL时处理板栗叶片,能提高上述保护性酶的活性,其中抗病品种北峪2号活性变化幅度最大,CAT、APX、PAL活性分别为对照的4.36、2.19、3.98倍;Cp-毒素处理浓度达到200μg/mL时,破坏了板栗叶片中的抗氧化酶系,酶活性明显下降,并显著低于对照。此外,抗病品种酶活性高于中抗品种和感病品种;防御酶的活性与品种抗病性之间呈正相关,毒素作用后防御酶活性的变化可作为衡量板栗抗病性强弱的指标。
In order to investigate the difference of Cp-Toxin stress responses of Chestnut to different varieties of chestnut, Leaf-round method was used to study the effects of different concentrations of Chestnut on the activities of superoxide dismutase (SOD), catalase Peroxidase (POD), ascorbate peroxidase (APX) and other defense enzyme activity. The results showed that when the concentration of Cp-toxin was lower than 100μg / mL, the activity of protective enzyme was increased, and the activity of resistant variety Beiyu 2 was the largest, and the activities of CAT, APX and PAL were 4.36 , 2.19 and 3.98 times respectively. When the concentration of Cp-toxin reached 200 μg / mL, the antioxidant enzyme activity in chestnut leaves was destroyed, and the enzyme activity was significantly decreased, which was significantly lower than that of the control. In addition, the enzyme activities of resistant varieties were higher than that of medium resistant varieties and susceptible varieties. The activity of defensive enzymes was positively correlated with the disease resistance of varieties, and the change of defensive enzyme activity after toxins could be used as an index to measure the disease resistance of chestnut .