论文部分内容阅读
目的探讨拘束应激诱发小鼠抗流感病毒能力低下与营养型鸡精(Essence of Chicken,EC)的改善作用。方法将雄性昆明小鼠随机分为正常对照组、病毒组、模型组(拘束应激+病毒)、阳性药物(利巴韦林,50 mg.kg-1)组、EC低、高剂量组(12,24 mL.kg-)1。除正常对照组及病毒组外,其余各组在给药的第2天将小鼠拘束负荷18 h,拘束恢复1 d后,除正常组外其他各组小鼠经鼻黏膜感染流感病毒。从感染病毒当天开始连续21d,记录各组小鼠的死亡数目及小鼠的平均存活时间(MDD);计算病毒感染第4天小鼠肺脏指数;观察EC对拘束应激小鼠免疫功能的影响。结果利巴韦林及EC能提高因拘束应激负荷降低的小鼠抗病毒感染能力,延长生存时间和改善肺脏指数。EC能增加拘束小鼠的胸腺指数、脾脏指数和脾淋巴细胞数目,改善脾淋巴细胞亚群Th(CD3+CD4+)(辅助性T淋巴细胞)/Ts(CD3+CD8+)(抑制性T淋巴细胞)的比值,提高脾自然杀伤(NK)细胞活性,从而改善拘束应激小鼠的免疫功能。结论 EC可以通过改善免疫功能来提高拘束应激小鼠的抗病毒能力。
Objective To investigate the effect of restrictive stress-induced mouse anti-influenza virus (RSV) abatement and Essence of Chicken (EC). Methods Male Kunming mice were randomly divided into normal control group, virus group, model group (restraint stress + virus), positive drug (ribavirin, 50 mg.kg-1), EC low and high dose group 12, 24 mL.kg-) 1. Except the normal control group and the virus group, the mice in other groups were restrained for 18 hours on the second day after administration, and were rescued for 1 day. All the mice except the normal group were infected with the influenza virus through the nasal mucosa. The number of deaths and the mean survival time (MDD) of mice in each group were recorded 21 days after the virus was infected. The lung index of mice on the 4th day after virus infection was calculated. The effects of EC on the immune function of restraint stress mice . Results Ribavirin and EC increased the antiviral ability of mice with restricted stress load, prolonged survival and improved lung index. EC can increase thymus index, spleen index and splenic lymphocyte number in restraint mice, and improve Th (CD3 + CD4 +) (helper T lymphocyte) / Ts (CD3 + CD8 +) (inhibitory T lymphocyte ) Ratio, increase natural killer (NK) cell activity of the spleen, thereby improving the immune function of restraint stress mice. Conclusion EC can improve the anti-virus ability of restraint stress mice by improving immune function.