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目的建立高效液相色谱荧光法测定加替沙星注射液的含量方法.方法 CLC-ODS柱(15mm×6.0mm, 5μm),流动相:0.05mol/L枸橼酸-乙腈(80∶20),三乙胺调pH3.0,流速1 mL*min-1,Ex=360nm, Em=465nm,样品稀释1000倍,进样10μL.结果加替沙星在4～32ng范围内线性关系良好,回收率(99.98±0.06)%,日内、日间RSD＜2%.结论该法简便、快速、准确,可用于测定加替沙星注射液的含量.“,”OBJECTIVE To develop an HPLC method with fluorescence detection for analysis of gatifloxacin(GTX) injection.METHOD The HPLC system consisted of CLC-ODS column（15cm×6.0mm, 5μm）and a RF-10AXL fluorescence detector. The mobile phase used composed of 0.05mol*L-1 citric acid-acetonitrile (80∶20, v/v), adjusted pH to 3.0 with triethylamine, and the flow rate was set at 1mL*min-1. The fluorescence detector was set at λex=360nm and λem=465 nm. GTX injection was diluted 1000 times with water, then 10μL was injected into HPLC System. RESULTS The method was linear at the GTX range from 4.0 ng to 32.0ng, the recovery of the method was (99.98±0.06)%(n=5), and the RSD was ＜2% for precision of intra-day and between-day.CONCLUSIONS The method is simple, rapid and accurate, can be used for determination of GTX.