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乙型肝炎病毒(HBV)和丙型肝炎病毒(HCV)感染肝细胞之后,肝炎病毒蛋白的表达对与肝细胞中正常的信号转导通路产生显著的影响.肝炎病毒蛋白对于肝细胞信号转导的影响,势必影响肝细胞的细胞周期、细胞凋亡、细胞生长以及细胞的恶性转化等环节.因为这些细胞的基本的生命活动现象,都与细胞正常的信号转导有关.肝炎病毒蛋白对肝细胞信号调节的影响,一方面通过蛋白一蛋白之间的相互作用而实现,另一方面也通过对肝细胞基因表达谱的影响而实现.因此,利用现代分子生物学技术,对肝炎病毒蛋白结合的蛋白基因进行研究,利用基因差异表达技术对肝炎病毒蛋白反式调节的靶基因进行筛选,阐明肝炎病毒蛋白表达时,能够上调和下调的靶基因类型,对于深入研究肝细胞获得肝炎病毒基因表达之后信号转导途径具有十分重要的意义.中国人民解放军第302医院传染病研究所基因治疗研究中心,在内科传染病学专业博士生导师、生物化学与分子生物学专业博士生导师成军博士、教授、主任医师的领导下,建立了筛选肝炎病毒调节基因结合蛋白的酵母单杂交技术,筛选肝炎病毒蛋白、肝细胞蛋白的结合蛋白的酵母双杂交技术,筛选肝炎病毒蛋白反式调节肝细胞基因的抑制性消减杂交和表达谱基因芯片研究技术等,系统研究肝炎病毒与肝细胞相互作用的分子生物学机制,并有新的发现.本专题研究中所发表的这些重要文章,对于进一步深入探索和研究肝炎病毒对肝细胞正常信号转导的影响,将产生重要推动作用.
The expression of hepatitis virus protein has a significant effect on normal signal transduction pathways in hepatocytes after Hepatitis B virus (HBV) and Hepatitis C virus (HCV) infection of hepatocytes.Hepatitis virus proteins have a profound effect on hepatocyte signaling , It is bound to affect the cell cycle of liver cells, apoptosis, cell growth and malignant transformation of cells and so on.Because these cells are the basic life activity phenomenon, and the normal signal transduction of cells.Hepatitis virus protein on the liver The effects of cell signaling are mediated through protein-protein interactions on the one hand, and liver-cell gene expression profiles on the other. Therefore, the use of modern molecular biology techniques for the binding of hepatitis virus proteins Of the genes of genes were studied, the use of gene differential expression of hepatitis B virus protein target gene trans-screening to clarify the hepatitis virus protein expression, up-and down-regulation of the target gene type, for in-depth study of hepatitis B virus gene expression After the signal transduction pathway has a very important significance.Chinese People’s Liberation Army 302 HospitalInfectious Institute of gene therapy research center, medical infectious disease science doctoral tutor, biochemistry and molecular biology doctoral tutor Cheng Jun doctor, professor, chief physician under the leadership of the establishment of the hepatitis B virus regulatory gene binding protein yeast Single hybridization, screening of hepatitis virus protein, hepatocyte protein binding protein yeast two-hybrid technology, screening of hepatitis virus protein trans-regulation of hepatocyte genes suppression subtractive hybridization and expression profiling gene chip research technology, systematic study of hepatitis virus and Molecular mechanism of liver cell interaction and new discoveries.These important articles published in this study will have an important impetus for further exploring and studying the influence of hepatitis virus on the normal signal transduction of hepatocytes .