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探讨脱氢枞胺衍生物DHAA-urea对有氧和乏氧状态的HepG2细胞的生长及糖代谢的影响。150μmol/L浓度氯化钴的无血清DMEM预培养24h致乏氧细胞模型,使用MTT法检测DHAA-urea(1～50μmol/L)分别对常氧和乏氧HepG2细胞的生长抑制情况。分别使用试剂盒检测DHAA-urea作用常氧与乏氧HepG2后细胞内ATP浓度,乳酸脱氢酶(LDH)以及葡萄糖-6-磷酸脱氢酶(G6PD)活性的变化。与正常对照组比较,DHAA-urea对常氧乏氧HepG2细胞的生长都具有明显的抑制作用,胞内ATP,LDH和G6PD含量与活性均受到抑制,且与DHAA-urea剂量和作用时间呈依赖性。DHAA-urea对HepG2细胞的生长抑制很可能与其抑制糖代谢导致能量耗竭相关。DHAA-urea是抗肿瘤领域较具发展潜力的新化合物。
To investigate the effect of dehydroabietylamine derivative DHAA-urea on the growth and glucose metabolism in HepG2 cells under aerobic and hypoxia conditions. The model of hypoxic cell was pretreated with serum-free DMEM with 150μmol / L cobalt chloride for 24 hours. MTT assay was used to detect the inhibitory effect of DHAA-urea (1 ~ 50μmol / L) on the growth of normoxic and hypoxic HepG2 cells respectively. The changes of intracellular ATP concentration, lactate dehydrogenase (LDH) and glucose-6-phosphate dehydrogenase (G6PD) activity of DHAA-urea treated with normoxia and hypoxia HepG2 were detected by kit respectively. Compared with the normal control group, DHAA-urea significantly inhibited the growth of normoxia-hypoxic HepG2 cells, and inhibited the content and activity of intracellular ATP, LDH and G6PD, and was dependent on the dose and duration of DHAA-urea Sex. The inhibitory effect of DHAA-urea on the growth of HepG2 cells is likely related to its inhibition of glucose metabolism leading to energy depletion. DHAA-urea is a new compound with potential for development in the field of anti-tumor.