探索脓毒症引起的急性肺损伤（ＡＬＩ）时内源性肺表面活性物质（ＰＳ）主要成分的变化。方法制作兔脓毒性ＡＬＩ模型，进行支气管肺泡灌洗液（ＢＡＬＦ）磷脂、功能亚型聚集体及活性的分析，Ｗｅｓｔｅｒｎｄｏｔｂｌｏｔ法和Ｎｏｒｔｈｅｒｎｂｌｏｔ法测肺表面活性物质结合蛋白（ＳＰＡ）含量及ｍＲＮＡ含量的变化。结果肺损伤后与正常组比，总磷脂（ＴＰＬ）（７．８±１．１）ｖｓ（１２．７±１．７）ｍｇ／ｋｇ，饱和磷脂占ＴＰＬ百分比（ＤＳＰＣ／ＴＰＬ）（２６．６±５．６）％ｖｓ（３５．７±４．８）％，ＳＰ－Ａ蛋白（８０．８±１８．２）ｖｓ（１７３．７±１５．５）μｇ／ｋｇ，ＳＰ－ＡｍＲＮＡ５０．３±８．９ｖｓ８２．１±１２．９，含量均显著减少。总蛋白（ＴＰ）（１００．９±７．３）ｖｓ（４９．３±２．７）ｍｇ／ｋｇ，小聚积体（ＳＡ）与大聚集体（ＬＡ）的比例（ＳＡ／ＬＡ）１．１１±０．１５ｖｓ０．５４±０．１，最小表面张力（γｍｉｎ）（２０．２±０．８）ｖｓ（８．３±５．９）ｍＮ／ｍ，均有显著的异常升高。结论脓毒性ＡＬＩ时内源性ＰＳ系统主要成分发生了质量和功能的异常损害，可能是ＡＬＩ时疾病过程难以控制的内在原因 To explore the changes of the main components of endogenous pulmonary surfactant (PS) in sepsis-induced acute lung injury (ALI). Methods Rabbit septic ALI model was made. The phospholipids of bronchoalveolar lavage fluid (BALF), the functional subtype aggregates and activities were analyzed. The contents of SPA and mRNA of SPA were measured by Westerndotblot and Northern blot. The change. Results Compared with the normal group, the total phospholipids (TPL) (7.8 ± 1.1) vs (12.7 ± 1.7) mg / kg and the percentage of TPL (% 6 ± 5.6% vs 35.7 ± 4.8%, SP-A protein 80.8 ± 18.2 vs 173.7 ± 15.5 μg / kg, SP-AmRNA50. 3 ± 8.9vs82.1 ± 12.9, the content was significantly reduced. Total protein (TP) was (100.9 ± 7.3) vs (49.3 ± 2.7) mg / kg, and the ratio of small aggregates (SA) to large aggregates (LA) 11 ± 0.15vs0.54 ± 0.1, the minimum surface tension (20min ± 0.8) vs (8.3 ± 5.9) mN / m, all showed significant abnormal increase. Conclusion The main components of endogenous PS system in septic ALI have abnormal quality and function damage, which may be the underlying reason for the disease process is difficult to control in ALI