应用微板核酸杂交 - EL ISA技术 ,按照 Okamoto等发表的基因序列 ,选取 TTV ORF1的保守序列作为内引物 ;包被探针选择一段 型、 型同源的序列 nt2 35 0 - nt2 495 ,而显色探针 和显色探针 则选择异源性大于 5 0 %的序列 ,并在显色探针的 5′端标上生物素。首先对患者及献血 According to the gene sequence published by Okamoto et al., The conserved sequence of TTV ORF1 was selected as the internal primer by using the microplate nucleic acid hybridization - ELISA technique. The probe was used to select a type and homologous sequence nt235 0 - nt2 495, Color probes and chromogenic probes are those that have a heteroplasmy greater than 50% and have biotin labeled on the 5 ’end of the chromogenic probe. First of all for patients and donate blood