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目前,黑热病的发病率有回升的趋势,受到国内外广泛重视。甘肃、四川两省自1983年发病人数逐年上升。其主要传染源均为病犬。犬的感染率高达8.5～2.5％。鉴于本实验室通过K-DNA分子杂交技术,发现杜氏利什曼原虫四川人分离株与犬分离株K-DNA微环碱基序列不完全同源,以及它们之间蛋白质组成分析的差异的存在,本科研为此制备出抗犬分离株前鞭毛体膜抗原单克隆抗体,并通过下述实验研究其保护性功能。
At present, the incidence of kala-azar has a tendency to pick up and is widely valued both at home and abroad. The incidence of the disease in Gansu and Sichuan provinces increased year by year since 1983. The main source of infection are dogs. Dog infection rate as high as 8.5 ~ 2.5%. In view of the K-DNA molecular hybridization technique in our laboratory, it was found that the Leishmania donovani Sichuan isolate was not completely homologous to the K-DNA microcyclic base sequence of the canine isolate and the difference in the protein composition analysis between them , This research to prepare anti-dog isolates of promastigote membrane antigen monoclonal antibody, and through the following experiments to study its protective function.