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腐植酸是风化煤的主要成分,一般水溶性和生理活性很低,直接应用效果很差,通过微生物处理有望提高其活性,开发风化煤应用新技术。本研究从土壤、根际、枯枝落叶、风化煤等多种生境中筛选到能利用风化煤或腐植酸的69个菌种,但仅有6个菌株能液化风化煤,出现小液珠或使培养基染成黄褐色至黑色。液体培养条件下,风化煤失重率达到47.69％(山西风化煤)和39.54％(内蒙古风化煤),紫外和红外光谱分析表明风化煤经微生物作用后,不仅腐植酸被溶解出来,同时被分解,分子中-OH增加、C=O基团减少,分子间缔合程度降低,更多基团暴露在外。
Humic acid is the main component of weathered coal, the general water solubility and low physiological activity, the direct application of poor results, through microbial treatment is expected to increase its activity, the development of weathering coal application of new technologies. In this study, 69 bacterial species that could utilize weathered coal or humic acid were screened from various habitats such as soil, rhizosphere, litter and weathered coal, but only 6 strains could liquefy weathered coal with small droplets or The medium is dyed in tan to black. Under liquid culture conditions, the weightlessness rate of weathered coal reached 47.69% (Shanxi weathered coal) and 39.54% (Inner Mongolia weathered coal). UV and FTIR analysis showed that not only humic acid was decomposed but also humic acid was weathered by weathered coal, In the molecule, -OH increases, the number of C = O groups decreases, the degree of intermolecular association decreases, and more groups are exposed.